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G anoderma lucidum is widely used in Asia to treat health problems and increase vitality and longevity. These mushrooms offer various health benefits, including immunomodulatory, antitumor, antibacterial, antiviral, anti-diabetic, and anti-oxidant properties [1, 2]. Ganoderic Acid (GA) is a type of triterpenoids produced by G. lucidum and is considered a secondary metabolite [3]. Some GAs can inhibit cholesterol synthesis and tumor growth, which can effectively cure cancer [4, 5]. However, due to the low efficacy of GA production from G. lucidum, these useful metabolite applications have been limited.

Therefore, investigations are growing to enhance the GA biosynthesis’ efficiency by evaluating diverse conditions, in desired parameters such as pH, oxygen pressure and shear stress [6-8]. Furthermore, several studies have been performed to find suitable culture media for optimizing the production of mycelial biomass and secondary metabolite. Although available studies suggests that the more complex media are superior in cell growth and production of secondary metabolites compared to other media, such as semi-synthetic [9], further research is needed to improve the efficacy of production. It has also been reported that the biomass and secondary metabolite yields are increased by improving the oxygen supply [10, 11]. On the other hand, before scale-up studies, the traditional cultivation process has required the screening of large numbers of samples, and consequently, a vast number of development cultivations should be performed. So far, many investigations have been done to enhance G. lucidum production; however, in all of these studies …