作者
Roger Pechous, Jean Celli, Renee Penoske, Stanley F Hayes, Dara W Frank, Thomas C Zahrt
发表日期
2006/8
期刊
Infection and immunity
卷号
74
期号
8
页码范围
4452-4461
出版商
American Society for Microbiology
简介
Francisella tularensis is a facultative intracellular pathogen and is the etiological agent of tularemia. It is capable of escaping from the phagosome, replicating to high numbers in the cytosol, and inducing apoptosis in macrophages of a variety of hosts. F. tularensis has received significant attention recently due to its potential use as a bioweapon. Currently, there is no licensed vaccine against F. tularensis, although a partially protective live vaccine strain (LVS) that is attenuated in humans but remains fully virulent for mice was previously developed. An F. tularensis LVS mutant deleted in the purMCD purine biosynthetic locus was constructed and partially characterized by using an allelic exchange strategy. The F. tularensis LVS ΔpurMCD mutant was auxotrophic for purines when grown in defined medium and exhibited significant attenuation in virulence when assayed in murine macrophages in vitro or in BALB/c …
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R Pechous, J Celli, R Penoske, SF Hayes, DW Frank… - Infection and immunity, 2006