作者
Hsun-Ming Chang, Christian Klausen, Peter CK Leung
发表日期
2013/8/1
期刊
Fertility and sterility
卷号
100
期号
2
页码范围
585-592. e1
出版商
Elsevier
简介
Objective
To investigate the effects of antimüllerian hormone (AMH) on basal and FSH-induced cytochrome P450 aromatase (aromatase) expression and E2 production in human granulosa-lutein (hGL) cells, and to elucidate the mechanism by which AMH exerts its effects.
Design
Experimental study.
Setting
Academic medical center for reproductive science.
Patient(s)
The hGL cells were obtained from consenting patients undergoing IVF treatment.
Intervention(s)
None.
Main Outcome Measure(s)
Primary cultures of hGL cells were used to examine the effects of AMH (10 ng/mL) on basal and FSH (0.2 IU/mL)-stimulated E2 and intracellular cyclic adenosine 3′:5′ monophosphate (cAMP) accumulation, as well as aromatase and FSH receptor expression. Small interfering RNA targeting type II AMH receptor (AMHR2) was used to verify the specificity of the effects.
Result(s)
Treatment with AMH significantly reduced FSH …
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HM Chang, C Klausen, PCK Leung - Fertility and sterility, 2013