作者
Hsun-Ming Chang, Lanlan Fang, Jung-Chien Cheng, Elizabeth L Taylor, Ying-Pu Sun, Peter CK Leung
发表日期
2016/2/1
期刊
Fertility and sterility
卷号
105
期号
2
页码范围
520-528
出版商
Elsevier
简介
Objective
To investigate the biological role of growth differentiation factor 8 (GDF8) in the regulation of steroidogenesis in human granulosa-lutein (hGL) cells.
Design
Experimental study.
Setting
Academic medical center.
Patient(s)
In vitro fertilization patients who provided hGL cells.
Intervention(s)
Cultured hGL cells treated with recombinant human GDF8 for 24 hours.
Main Outcome Measure(s)
Expression of steroidogenic enzymes and steroid production in primary cultures of hGL cells used to investigate the effects of GDF8 via specific mRNA and protein levels examined using real time-quantitative polymerase chain reaction and Western blot analysis, respectively, and levels of estradiol and progesterone measured by enzyme immunoassays.
Result(s)
Extracts were prepared from cultured hGL cells after exposure to GDF8. The levels of cytochrome P450 aromatase (aromatase), the FSH receptor, and estradiol were …
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HM Chang, L Fang, JC Cheng, EL Taylor, YP Sun… - Fertility and sterility, 2016