作者
Shahla Roudbarmohammadi, Maryam Roudbary, Bita Bakhshi, Farzad Katiraee, Rasoul Mohammadi, Mehraban Falahati
发表日期
2016/1/1
期刊
Advanced biomedical research
卷号
5
期号
1
页码范围
105
出版商
Medknow
简介
Background:
A cluster of genes are involved in the pathogenesis and adhesion of Candida albicans to mucosa and epithelial cells in the vagina, the important of which is agglutinin-like sequence (ALS) genes. As well as vaginitis is a significant health problem among women, the antifungal resistance of Candida species is continually increasing. This cross-sectional study investigates the expression of ALS1 and ALS3 genes and biofilm formation in C. albicans isolate isolated from vaginitis.
Materials and Methods:
Fifty-three recognized isolates of C. albicans were collected from women with recurrent vulvovaginal candidiasis in Iran, cultured on sabouraud dextrose agar, and then examined for gene expression. Total messenger RNA (mRNA) extracted from C. albicans isolates and complementary DNA synthesized using reverse transcriptase enzyme. Reverse transcription-polymerase chain reaction (RT-PCR) using …
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S Roudbarmohammadi, M Roudbary, B Bakhshi… - Advanced biomedical research, 2016