查看文章

Methods

Methods

The 7 leading scientific journals by citation impact factor (Journal Citation Reports, Thomson Scientific, Philadelphia, Pa, 2004) that regularly publish original animal studies were searched: Science, Nature, Cell, Nature Medicine, Nature Genetics, Nature Immunology, and Nature Biotechnology. Articles with more than 500 citations were retrieved under the assumption that such prominent findings would more likely be tested in subsequent human trials. 4 A total of 2000 articles published between 1980 and 2000 were screened, reflecting advances in molecular biology and recombinant genetics. Articles were included if they investigated a preventive or therapeutic intervention in an in vivo animal model. When there were multiple animal studies of the same intervention, the most cited study was retained. Power calculations (α= 0.05, β= 0.05) estimated that 49 articles were needed to exclude a …