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A novel molecularly imprinted polymer (MIP) electrochemical sensor for the sensitive detection of lomefloxacin (LFX) was developed for the first time. For this, Fe-doped porous carbon (Fe-PC) was firstly synthesized and then used to modify the Au electrode. MIP film with binary functional monomers was fabricated on the Fe-PC modified Au electrode surface by electropolymerization, with LFX as template molecule, o-phenylenediamine (o-PD) and β-cyclodextrin (β-CD) as binary functional monomers. The results showed that the developed sensor exhibited high selectivity and sensitivity in detection of LFX, which was attributed to the large specific surface area, rich porosity and good catalytic property of Fe-PC, as well as high selectivity and affinity of MIP prepared with binary functional monomers. Under the optimal conditions, the current response was linear to the concentration of LFX ranging from 1 to 120 nM …