作者
Nanyoung Yoon, Seunggyu Kim, Hye Kyoung Sung, Thanh Q Dang, Jessie S Jeon, Gary Sweeney
发表日期
2021/2/1
期刊
Biochimica et Biophysica Acta (BBA)-General Subjects
卷号
1865
期号
2
页码范围
129796
出版商
Elsevier
简介
Background
Iron excess is a risk factor for cardiovascular diseases and it is important to understand the effect of iron on vascular permeability, particularly for the transport of large metabolic hormones such as adiponectin.
Methods
We used 2-dimensional monolayers of cultured human dermal microvascular endothelial cells (HDMEC) and human umbilical vein endothelial cells (HUVEC) as well as 3-dimensional microvascular networks to measure transendothelial flux.
Results
Iron supplementation reduced transendothelial electric resistance (TEER). Flux analysis indicated that under control conditions permeability of 70 kDa dextran and oligomeric forms of adiponectin were restricted in comparison with a 3 kDa dextran, however upon iron treatment permeability of the larger molecules was increased. The increased permeability and size-dependent trans-endothelial movement in response to iron was also observed …
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N Yoon, S Kim, HK Sung, TQ Dang, JS Jeon… - Biochimica et Biophysica Acta (BBA)-General Subjects, 2021