作者
Siying Wen, Guanghong Zhou, Shangxin Song, Xinglian Xu, Josef Voglmeir, Li Liu, Fan Zhao, Mengjie Li, Li Li, Xiaobo Yu, Yun Bai, Chunbao Li
发表日期
2015/11
期刊
Proteomics
卷号
15
期号
21
页码范围
3688-3698
简介
In vitro digestion products of proteins were compared among beef, pork, chicken, and fish. Gastric and jejunal contents from the rats fed these meat proteins were also compared. Cooked pork, beef, chicken, and fish were homogenized and incubated with pepsin alone or followed by trypsin. The digestion products with molecular weights of less than 3000 Da were identified with MALDI‐TOF‐MS and nano‐LC‐MS/MS. Gastric and jejunal contents obtained from the rats fed the four meat proteins for 7 days were also analyzed. After pepsin digestion, pork, and beef samples had a greater number of fragments in similarity than chicken and fish samples, but the in vitro digestibility was the greatest (p < 0.05) for pork and the smallest for beef samples. After trypsin digestion, the species differences were less pronounced (p > 0.05). A total of 822 and 659 peptides were identified from the in vitro and in vivo digestion products …
学术搜索中的文章
S Wen, G Zhou, S Song, X Xu, J Voglmeir, L Liu… - Proteomics, 2015