作者
Yongming Wang, Wendy Y Zhang, Shijun Hu, Feng Lan, Andrew S Lee, Bruno Huber, Leszek Lisowski, Ping Liang, Mei Huang, Patricia E de Almeida, Jong H Won, Ning Sun, Robert C Robbins, Mark A Kay, Fyodor D Urnov, Joseph C Wu
发表日期
2012/12/7
期刊
Circulation research
卷号
111
期号
12
页码范围
1494-1503
出版商
Lippincott Williams & Wilkins
简介
Rationale:
Molecular imaging has proven to be a vital tool in the characterization of stem cell behavior in vivo. However, the integration of reporter genes has typically relied on random integration, a method that is associated with unwanted insertional mutagenesis and positional effects on transgene expression.
Objective:
To address this barrier, we used genome editing with zinc finger nuclease (ZFN) technology to integrate reporter genes into a safe harbor gene locus (PPP1R12C, also known as AAVS1) in the genome of human embryonic stem cells and human induced pluripotent stem cells for molecular imaging.
Methods and Results:
We used ZFN technology to integrate a construct containing monomeric red fluorescent protein, firefly luciferase, and herpes simplex virus thymidine kinase reporter genes driven by a constitutive ubiquitin promoter into a safe harbor locus for fluorescence imaging, bioluminescence …
学术搜索中的文章
Y Wang, WY Zhang, S Hu, F Lan, AS Lee, B Huber… - Circulation research, 2012