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This study investigated the sources of inoculum of Botyosphaeriaceae species in three commercial grapevine nurseries in New Zealand. Botryosphaeriaceae propagules were detected on the surfaces of 33 to 100 % of canes and 33 % of dead grapevine materials using microscopy, plating assays and PCR with Botryosphaeriaceae multi-species primers (Bot100F/Bot472R). Isolations also showed that 15 to 68 % of the canes were internally infected by Botryosphaeriaceae species, with Neofusicoccum luteum and N. parvum being the most predominant species. Incidence varied between nurseries, with Nursery 5 having highest N. luteum incidence (53 %) and Nursery 3 having highest N. parvum incidence (88 %). Botryosphaeriaceae DNA was detected in rain-water run-off from the mother-vine canopy, but not in soil samples collected around infected mother-vines. However, samples from the nursery …