作者
Michael Mor, Michal Werbner, Joel Alter, Modi Safra, Elad Chomsky, Smadar Hada-Neeman, Ksenia Polonsky, Cameron J Nowell, Alex E Clark, Anna Roitburd-Berman, Noam Ben Shalom, Michal Navon, Dor Rafael, Hila Sharim, Evgeny Kiner, Eric Griffis, Jonathan M Gershoni, Oren Kobiler, Sandra Lawrynowicz Leibel, Oren Zimhony, Aaron F Carlin, Gur Yaari, Moshe Dassau, Meital Gal-Tanamy, David Hagin, Ben A Croker, Natalia T Freund
发表日期
2020/10/6
期刊
BioRxiv
出版商
Cold Spring Harbor Laboratory Preprints
简介
The interactions between antibodies, SARS-CoV-2 and immune cells contribute to the pathogenesis of COVID-19 and protective immunity. To understand the differences between antibody responses in mild versus severe cases of COVID-19, we analyzed the B cell responses in patients 1.5 months post SARS-CoV-2 infection. Severe and not mild infection correlated with high titers of IgG against Spike receptor binding domain (RBD) that were capable of viral inhibition. B cell receptor (BCR) sequencing revealed two VH genes, VH3–38 and VH3–53, that were enriched during severe infection. Of the 22 antibodies cloned from two severe donors, six exhibited potent neutralization against live SARS-CoV-2, and inhibited syncytia formation. Using peptide libraries, competition ELISA and RBD mutagenesis, we mapped the epitopes of the neutralizing antibodies (nAbs) to three different sites on the Spike. Finally, we …
学术搜索中的文章
M Mor, M Werbner, J Alter, M Safra, E Chomsky… - BioRxiv, 2020