作者
David Zemmour, Evgeny Kiner, Christophe Benoist
发表日期
2020/4/1
来源
Current opinion in immunology
卷号
63
页码范围
61-67
出版商
Elsevier Current Trends
简介
Highlights
- Single-cell transcriptomics can reveal unrecognized heterogeneity and CD4 T subsets.
- An unexpected CD4+ T population with high levels of IFN-I responsive transcripts.
- Previously defined CD4 subsets more complex than anticipated.
- The end of Thx: continuous, rather than discrete, distribution of key cytokines.
- Phenotypes are driven by TCR signals, but not solely.
Single-cell transcriptomics (scRNAseq) holds the promise to generate definitive atlases of cell types. We review scRNAseq studies of conventional CD4+ αβ T cells performed in a variety of challenged contexts (infection, tumor, allergy) that aimed to parse the complexity and representativity of previously defined CD4+ T cell types, lineages, and cosmologies. With a few years’ experience, the field has realized the difficulties and pitfalls of scRNAseq. With the very high-dimensionality of scRNAseq data, subset definitions based on low …
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D Zemmour, E Kiner, C Benoist - Current opinion in immunology, 2020