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In our data-rich world, data literacy is becoming an increasingly important goal in education as data sets become bigger, and more complex. However, data literacy falls outside the traditional topic domain of secondary science curricula and so may be beyond the scope of the pedagogical content knowledge (PCK) of teachers who have received training in only one subject area. This paper presents an exploratory study of how secondary school science teachers learn and use the content and skills of integrating data literacy into their curriculum, within the context of a PD program for bioinformatics. The findings suggest that there are a separate set of PCK components that teachers need to master in order to successfully teach data literacy, and that successful PD must support teachers in developing them.