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Anaplasmosis, babesiosis, and heartwater are the three most important tick-borne diseases of cattle in South Africa and result in a large number of mortalities. Endemic stability contributes to disease control, but little is known about the conditions required for maintenance of endemic stability. Through the on-going Health and Demographic Surveillance System in Livestock in the study area of the Mnisi One Health Platform, Mpumalanga, a great deal of information is being collected about cattle in the area, with the eventual aim of developing mathematical models to describe and predict infections. More than 15000 cattle have been identified for tick burden assessment, serological analyses and parasite identification. However, little is known about the time-course of infection of cattle with various tick-borne haemoparasites. Therefore, this study aimed to investigate the time-course of infection in new-born calves (n …