作者
Nicolas A Giraldo, Etienne Becht, Yann Vano, Florent Petitprez, Laetitia Lacroix, Pierre Validire, Rafael Sanchez-Salas, Alexandre Ingels, Stephane Oudard, Audrey Moatti, Benedicte Buttard, Sarah Bourass, Claire Germain, Xavier Cathelineau, Wolf H Fridman, Catherine Sautès-Fridman
发表日期
2017/8/1
期刊
Clinical Cancer Research
卷号
23
期号
15
页码范围
4416-4428
出版商
American Association for Cancer Research
简介
Purpose: The efficacy of PD-1 checkpoint blockade as adjuvant therapy in localized clear cell renal cell carcinoma (ccRCC) is currently unknown. The identification of tumor microenvironment (TME) prognostic biomarkers in this setting may help define which patients could benefit from checkpoint blockade and uncover new therapeutic targets.
Experimental Design: We performed multiparametric flow cytometric immunophenotypic analysis of T cells isolated from tumor tissue [tumor-infiltrating lymphocytes (TIL)], adjacent non-malignant renal tissue [renal-infiltrating lymphocytes (RIL)], and peripheral blood lymphocytes (PBL), in a cohort of patients (n = 40) with localized ccRCC. Immunophenotypic data were integrated with prognostic and histopathologic variables, T-cell receptor (TCR) repertoire analysis of sorted CD8+PD-1+ TILs, tumor mRNA expression, and digital quantitative …
学术搜索中的文章
NA Giraldo, E Becht, Y Vano, F Petitprez, L Lacroix… - Clinical Cancer Research, 2017