作者
Weifeng Zhang, Lianyan Wang, Tingyuan Yang, Yuan Liu, Xiaoming Chen, Qi Liu, Jilei Jia, Guanghui Ma
发表日期
2015/9
期刊
Pharmaceutical research
卷号
32
页码范围
2837-2850
出版商
Springer US
简介
Purpose
Adjuvants are required to ensure the efficacy of subunit vaccines. Incorporating molecular immunopotentiators within particles could overcome drawbacks of molecular adjuvants (such as solubility and toxicity), and improve adjuvanticity of particles, achieving stronger adjuvant activity. Aim of this study is to evaluate the adjuvanticity of immunopotentiator-loaded polymeric particles for subunit vaccine.
Methods
PLGA microparticles (PMPs) and imiquimod (TLR-7 ligand)-loaded PLGA microparticles (IPMPs) were prepared by SPG premix membrane emulsification. In vitro and in vivo studies were performed to their adjuvant activity, using ovalbumin and H5N1 influenza split vaccine as antigens.
Results
Incorporating imiquimod into microparticles significantly improved the efficacy of PLGA microparticles in activating …
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