作者
Gabriele Vargas, Juliana DB Rocha, Debora Leite Oliveira, Priscila Costa Albuquerque, Susana Frases, Suelen S Santos, Joshua Daniel Nosanchuk, Andre Marco Oliveira Gomes, Lia CAS Medeiros, Kildare Miranda, Tiago JP Sobreira, Ernesto S Nakayasu, Emma A Arigi, Arturo Casadevall, Allan J Guimaraes, Marcio L Rodrigues, Celio Geraldo Freire‐de‐Lima, Igor C Almeida, Leonardo Nimrichter
发表日期
2015/3
期刊
Cellular microbiology
卷号
17
期号
3
页码范围
389-407
简介
The release of extracellular vesicles (EV) by fungal organisms is considered an alternative transport mechanism to trans‐cell wall passage of macromolecules. Previous studies have revealed the presence of EV in culture supernatants from fungal pathogens, such as Cryptococcus neoformans, Histoplasma capsulatum, Paracoccidioides brasiliensis, Sporothrix schenckii, Malassezia sympodialis and Candida albicans. Here we investigated the size, composition, kinetics of internalization by bone marrow‐derived murine macrophages (MO) and dendritic cells (DC), and the immunomodulatory activity of C. albicans EV. We also evaluated the impact of EV on fungal virulence using the Galleria mellonella larvae model. By transmission electron microscopy and dynamic light scattering, we identified two populations ranging from 50 to 100 nm and 350 to 850 nm. Two predominant seroreactive proteins (27 kDa and …
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G Vargas, JDB Rocha, DL Oliveira, PC Albuquerque… - Cellular microbiology, 2015