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Amniotic fluid-derived stem (AFS) cells possess properties found also in mesenchymal stem cells (MSCs) such as multipotent differentiation, immunomodulatory activity and the lack of significant immunogenicity. MSCs have shown therapeutic potential for repair and regeneration of tissues damaged by injury or disease. In particular, MSC treatment of acute and chronic wounds result in accelerated wound closure, increased epithelialization, granulation tissue and angiogenesis. Given that AFS cells can be obtained less invasively than MSCs and show greater proliferative capacity in culture, we investigated whether they could augment wound healing in a similar fashion as MSCs. We used a murine skin lesion model and bioprinting technology to deliver AFS cells in a fibrin-collagen hydrogel. We hypothesize that AFS cells will have several advantages over MSCs for large-scale wound healing, and that AFS cells …