作者
Shuai Wang, Cailin Lei, Jiulin Wang, Jian Ma, Sha Tang, Chunlian Wang, Kaijun Zhao, Peng Tian, Huan Zhang, Changyan Qi, Zhijun Cheng, Xin Zhang, Xiuping Guo, Linglong Liu, Chuanyin Wu, Jianmin Wan
发表日期
2017/2/15
期刊
Journal of Experimental Botany
卷号
68
期号
5
页码范围
899-913
出版商
Oxford University Press
简介
Lesion-mimic mutants are useful to dissect programmed cell death and defense-related pathways in plants. Here we identified a new rice lesion-mimic mutant, spotted leaf 33 (spl33) and cloned the causal gene by a map-based cloning strategy. SPL33 encodes a eukaryotic translation elongation factor 1 alpha (eEF1A)-like protein consisting of a non-functional zinc finger domain and three functional EF-Tu domains. spl33 exhibited programmed cell death-mediated cell death and early leaf senescence, as evidenced by analyses of four histochemical markers, namely H2O2 accumulation, cell death, callose accumulation and TUNEL-positive nuclei, and by four indicators, namely loss of chlorophyll, breakdown of chloroplasts, down-regulation of photosynthesis-related genes, and up-regulation of senescence-associated genes. Defense responses were induced in the spl33 mutant, as shown by enhanced …
学术搜索中的文章
SPL33, encoding an eEF1A-like protein, negatively regulates cell death and defense responses in rice
S Wang, C Lei, J Wang, J Ma, S Tang, C Wang, K Zhao… - Journal of Experimental Botany, 2017