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Worldwide demand for clean and safe drinking water is a serious concern due to sharply rising industrialization and the growing population. In this work, Spirulina (microalgae) based green synthesis of mesoporous iron oxide nanoparticles (MIONPs) was performed for the adsorption of chromium (VI) in an aqueous solution. Synthesized MIONPs were characterized by XRD, FT-IR, Raman Spectroscopy, Zeta Potential, Particle Size Analyzer, and FESEM with EDX. The mesoporous nature of synthesized material was confirmed using Brunauer–Emmett–Teller (BET) with a pore diameter of 3.837 nm. The adsorption mechanism of Chromium (VI) by synthesized MIONPs was explained using XPS analysis. The various other parameters including pH, Temperature, ion concentration, adsorbent dose, and contact time were also studied in batch experiments for chromium removal. The synthesized material shows the best …