作者
Nazli Akin, Dulama Richani, Xiuhua Liao, Yiqing Zhao, Anamaria-Cristina Herta, Katy Billooye, William A Stocker, David G Mottershead, Craig A Harrison, Johan Smitz, Ellen Anckaert, Robert B Gilchrist
发表日期
2022/1/1
期刊
Journal of Assisted Reproduction and Genetics
页码范围
1-14
出版商
Springer US
简介
Purpose
In vitro maturation (IVM) is a technology that generates mature oocytes following culture of immature cumulus-oocyte complexes (COC) in vitro. IVM is characterized by minimal patient stimulation, making it attractive for certain patient groups. Recently, a biphasic IVM system, capacitation (CAPA)-IVM, has shown improved clinical outcomes relative to standard IVM; however, it remains less efficient than IVF. This study assessed whether supplementation of CAPA-IVM culture media with the novel TGFβ superfamily proteins cumulin and super-GDF9 improves subsequent mouse embryo development.
Methods
Immature mouse COCs were cultured by standard IVM or biphasic IVM ± cumulin or super-GDF9.
Results
Both cumulin and super-GDF9 in standard IVM significantly improved day-6 blastocyst rate (53.9% control, 73.6 …
学术搜索中的文章
N Akin, D Richani, X Liao, Y Zhao, AC Herta, K Billooye… - Journal of Assisted Reproduction and Genetics, 2022