作者
Nathalie Müller, Michaela Leroch, Julia Schumacher, David Zimmer, Anne Könnel, Klaus Klug, Thomas Leisen, David Scheuring, Frederik Sommer, Timo Mühlhaus, Michael Schroda, Matthias Hahn
发表日期
2018/8
期刊
New Phytologist
卷号
219
期号
3
页码范围
1062-1074
简介
- The Botrytis cinerea VELVET complex regulates light‐dependent development and virulence. The goal of this study was to identify common virulence defects of several VELVET mutants and to reveal their molecular basis.
- Growth, differentiation, physiology, gene expression and infection of fungal strains were analyzed, and quantitative comparisons of in planta transcriptomes and secretomes were performed.
- VELVET mutants showed reduced release of citric acid, the major acid secreted by the wild‐type, whereas no significant role for oxalic acid was observed. Furthermore, a common set of infection‐related and secreted proteins was strongly underexpressed in the mutants. Quantitative secretome analysis with 15N metabolic labeling revealed a correlation of changes in protein and mRNA levels between wild‐type and mutants, indicating that transcript levels determine the abundance of secreted proteins …
学术搜索中的文章
N Müller, M Leroch, J Schumacher, D Zimmer… - New Phytologist, 2018