作者
Gopal Kushawah, Luis Hernandez-Huertas, Joaquin Abugattas-Nuñez Del Prado, Juan R Martinez-Morales, Michelle L DeVore, Huzaifa Hassan, Ismael Moreno-Sanchez, Laura Tomas-Gallardo, Alejandro Diaz-Moscoso, Dahiana E Monges, Javier R Guelfo, William C Theune, Emry O Brannan, Wei Wang, Timothy J Corbin, Andrea M Moran, Alejandro Sánchez Alvarado, Edward Málaga-Trillo, Carter M Takacs, Ariel A Bazzini, Miguel A Moreno-Mateos
发表日期
2020/9/28
期刊
Developmental Cell
卷号
54
期号
6
页码范围
805-817. e7
出版商
Elsevier
简介
Early embryonic development is driven exclusively by maternal gene products deposited into the oocyte. Although critical in establishing early developmental programs, maternal gene functions have remained elusive due to a paucity of techniques for their systematic disruption and assessment. CRISPR-Cas13 systems have recently been employed to degrade RNA in yeast, plants, and mammalian cell lines. However, no systematic study of the potential of Cas13 has been carried out in an animal system. Here, we show that CRISPR-RfxCas13d (CasRx) is an effective and precise system to deplete specific mRNA transcripts in zebrafish embryos. We demonstrate that zygotically expressed and maternally provided transcripts are efficiently targeted, resulting in a 76% average decrease in transcript levels and recapitulation of well-known embryonic phenotypes. Moreover, we show that this system can be used in …
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G Kushawah, L Hernandez-Huertas, JAN Del Prado… - Developmental Cell, 2020