作者
Payal Agarwal, Melissa P Crepps, Natalie A Stahr, Will P Kretzschmar, Hannah C Harris, Nripesh Prasad, Shawn E Levy, Bruce F Smith
发表日期
2021/12/1
期刊
Biochemistry and biophysics reports
卷号
28
页码范围
101106
出版商
Elsevier
简介
Delay in cancer diagnosis often results in metastasis and an inability to successfully treat the tumor. The use of broadly cancer-specific biomarkers at an early stage may improve cancer treatment and staging. This study has explored circulatory exosomal miRNAs as potential diagnostic biomarkers to identify cancer patients. Secretory exosomal miRNAs were isolated from 13 canine cancer cell lines (lymphoma, mast cell tumor, histiocytic cell line, osteosarcoma, melanoma, and breast tumor) and were sequenced by Next-Generation sequencing (NGS). We have identified 6 miRNAs (cfa-miR-9, -1841, −1306, −345, −132, and −26b) by NGS that were elevated in all cancer cell types. The miRNAs identified by NGS were then examined by Q-RT-PCR. The PCR data demonstrated similar expression patterns to those seen with NGS but provided fold differences that were much lower than those seen for NGS. Cfa-miR-9 …
学术搜索中的文章
P Agarwal, MP Crepps, NA Stahr, WP Kretzschmar… - Biochemistry and biophysics reports, 2021