作者
Konstantinos A Papadakis, John Prehn, Sofia T Moreno, Lorna Cheng, Elias A Kouroumalis, Richard Deem, Tim Breaverman, Paul D Ponath, David P Andrew, Peter HR Green, Martin R Hodge, Scott W Binder, Stephan R Targan
发表日期
2001/8/1
期刊
Gastroenterology
卷号
121
期号
2
页码范围
246-254
出版商
WB Saunders
简介
Background & Aims
Thymus-expressed chemokine (TECK) or CCL25) is selectively expressed in the small bowel (SB), where lamina propria lymphocytes (LPL) and intraepithelial leukocyte expressing the cognate chemokine receptor CCR9 predominate. We characterize the role of TECK and CCR9-expresing lymphocytes in small intestinal Crohn's disease.
Methods
CCR9 expression on lymphocytes from lamina propria, mesenteric lymph node, and peripheral blood was analyzed by flow cytometry and by Northern blotting for LPL. TECK expression was analyzed in inflamed SB and colon by reverse-transcription polymerase chain reaction and immunohistochemistry.
Results
The fraction of CCR9+ T cells in inflamed SB was significantly lower than in uninvolved SB mucosa. In contrast, in peripheral blood lymphocytes, CCR9+ lymphocytes were markedly elevated in patients with small bowel Crohn's or celiac …
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KA Papadakis, J Prehn, ST Moreno, L Cheng… - Gastroenterology, 2001