作者
Qi T Yu, Masayuki Saruta, Armine Avanesyan, Phillip R Fleshner, Alison H Banham, Konstantinos A Papadakis
发表日期
2007/2/1
期刊
Inflammatory bowel diseases
卷号
13
期号
2
页码范围
191-199
出版商
Oxford University Press
简介
Background
CD4+CD25+ regulatory T cells (TR) can prevent or treat experimental murine colitis but little is known about their potential role in human inflammatory bowel disease (IBD). FOXP3 is a transcription factor that plays a critical role in the development and function of CD4+CD25+ TR. The aim of this study was to examine the presence and functional characteristics of TR cells in colonic lymphoid tissues in patients with ulcerative colitis (UC).
Methods
FOXP3 expression was assessed by flow cytometry, immunohistochemistry, and reverse-transcriptase polymerase chain reaction (RT-PCR). Functional characterization of CD4+CD25+ cells was analyzed by suppression of proliferation and secretion of cytokines by cocultured effector CD4+CD25− T cells.
Results
FOXP3+CD4+ T cells are increased in the lamina propria (LP) of inflamed and noninflamed …
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QT Yu, M Saruta, A Avanesyan, PR Fleshner… - Inflammatory bowel diseases, 2007