查看文章

Herein, we developed and characterized robust agarose–chitosan hydrogel using N‑hydroxysuccinimide (NHS) as a mild chemical cross-linker. The hydrogel offered a simple, effective and eco-friendlier support material with >90% of immobilization efficiency of horseradish peroxidase. The surface morphology and functional properties of the agarose–chitosan hydrogel with and without immobilized horseradish peroxidase were investigated by scanning electron microscopy and Fourier-transform infrared, respectively. The agarose–chitosan hydrogel-immobilized horseradish peroxidase (ACH–HRP) exhibited wide-working pH and temperature stability, and promising reusability for its substrate oxidation. The ACH-HRP preserved a better activity under acidic environments, pH 4.0 (38 vs. 5.9%), and well stabilized under alkaline conditions, retaining a 3.9-folds greater activity than a free counterpart at pH 10. With …