作者
Karina Hettwer, Martin Jähne, Kirstin Frost, Martin Giersberg, Gotthard Kunze, Michael Trimborn, Martin Reif, Jochen Türk, Linda Gehrmann, Freddy Dardenne, Femke De Croock, Marion Abraham, Anne Schoop, Joanna J Waniek, Thomas Bucher, Eszter Simon, Etienne Vermeirssen, Anett Werner, Karin Hellauer, Ursula Wallentits, Jörg E Drewes, Detlef Dietzmann, Edwin Routledge, Nicola Beresford, Tamara Zietek, Margot Siebler, Anne Simon, Helena Bielak, Henner Hollert, Yvonne Müller, Maike Harff, Sabrina Schiwy, Kirsten Simon, Steffen Uhlig
发表日期
2018/4/15
期刊
Science of the Total Environment
卷号
621
页码范围
612-625
出版商
Elsevier
简介
Endocrine-active substances can adversely impact the aquatic ecosystems. A special emphasis is laid, among others, on the effects of estrogens and estrogen mimicking compounds. Effect-based screening methods like in vitro bioassays are suitable tools to detect and quantify endocrine activities of known and unknown mixtures.
This study describes the validation of the Arxula-Yeast Estrogen Screen (A-YES®) assay, an effect-based method for the detection of the estrogenic potential of water and waste water. This reporter gene assay, provided in ready to use format, is based on the activation of the human estrogen receptor alpha. The user-friendly A-YES® enables inexperienced operators to rapidly become competent with the assay.
Fourteen laboratories from four countries with different training levels analyzed 17β-estradiol equivalent concentrations (EEQ) in spiked and unspiked waste water effluent and …
学术搜索中的文章
K Hettwer, M Jähne, K Frost, M Giersberg, G Kunze… - Science of the Total Environment, 2018
