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Somatic embryogenesis receptor kinases (SERKs) belong to the leucine rich repeat-receptor like kinase (LRR-RLK) subfamily, and many LRR-RLKs have been proven to play a key role in plant immune signal transmission. However, the functions of SERKs in resistance to stripe rust caused by Puccinia striiformis f. sp. tritici remains unknown. Here, we identified a gene, TaSERK1, from Xiaoyan 6 (XY6), a wheat cultivar possessing high-temperature seedling-plant (HTSP) resistance to the fungal pathogen P. striiformis f. sp. triticiand its resistance at seedling stage. The expression level of TaSERK1 was up-regulated upon P. striiformis f. sp. tritici inoculation under relatively high temperatures. The transcriptional level of TaSERK1 was significantly increased under exogenous salicylic acid (SA) and brassinosteroids (BR) treatments. Barley stripe mosaic virus (BSMV)-induced gene silencing assay indicated that TaSERK1 positively regulated the HTSP resistance to stripe rust. The transient expression of TaSERK1 in tobacco leaves confirmed its subcellular localization on plasma membrane. Furthermore, TaSERK1 interacted with and phosphorylated the chaperone protein TaDJA7, which belongs to the heat shock protein 40 (Hsp40) subfamily. Silencing TaDJA7 compromised the HTSP resistance to stripe rust. The results indicated that when the membrane immune receptor TaSERK1 perceives the dual signals from P. striiformis f. sp. tritici infection under relatively high temperatures, it transmits the signal to TaDJA7 to activate HTSP resistance to the pathogen.