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An air-cathode microbial fuel cell (MFC) was implemented to examine the cathode material: bismuth ferrite (BFO) nanoparticles mixed with carbon soot. The physicochemical characterization of prepared carbon soot showed that the carbon soot particles were porous and graphitic consisting of 88% carbon. The physicochemical characterization of BFO confirms that the nanoparticles are spherical, have a high surface area, and are electrochemically active. Based on the results of the linear sweep voltammetry (LSV) examination, the large surface area of BFO has been accounted for by high ORR (oxidation–reduction reaction) activities. The increasing loading rates of BFO (0.25–1 mg/cm²) showed increasing power output, with 1 mg/cm² achieving the maximum power density and current density (11.99 W/m³ and 35.45 A/m³, respectively), which were comparable to the power output of platinum. According to …