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The present study evaluated levels of polymerization of deoxygenated sickle hemoglobin molecules (poly-dHbS-M) in the presence of fractionated leaf extracts of Anacardium occidentale Linn., Psidium guajava Linn., and Terminalia catappa Linn in vitro as well as identified, quantified, and characterized the phytocomponents from fractionated leaf extracts that exhibited comparatively high potency to impede poly-dHbS-M. Non-hemolyzed sickle erythrocytes were premixed with 40, 60, and 80 mg/100 mL of each of the separate fractionated leaf extracts of A. occidentale, P. guajava, and T. catappa in phosphate-buffered saline (PBS; pH = 7.4), osmotically equivalent to 9.0 g/L NaCl. Poly-dHbS-M was induced by adding 2.0 g/100 mL Na₂S₂O₅ to the erythrocyte suspension. The absorbance of the erythrocyte suspension was measured at regular intervals of 30 s for 180 s. Identification, quantification, and …