作者
Pavel Neuzil, Chunyan Zhang, Juergen Pipper, Sharon Oh, Lang Zhuo
发表日期
2006/1/1
期刊
Nucleic acids research
卷号
34
期号
11
页码范围
e77-e77
出版商
Oxford University Press
简介
We have designed, fabricated and tested a real-time PCR chip capable of conducting one thermal cycle in 8.5 s. This corresponds to 40 cycles of PCR in 5 min and 40 s. The PCR system was made of silicon micromachined into the shape of a cantilever terminated with a disc. The thin film heater and a temperature sensor were placed on the disc perimeter. Due to the system's thermal constant of 0.27 s, we have achieved a heating rate of 175°C s−1 and a cooling rate of −125°C s−1. A PCR sample encapsulated with mineral oil was dispensed onto a glass cover slip placed on the silicon disc. The PCR cycle time was then determined by heat transfer through the glass, which took only 0.5 s. A real-time PCR sample with a volume of 100 nl was tested using a FAM probe. As the single PCR device occupied an area of only a few square millimeters, devices could be combined into a parallel system to increase …
引用总数
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P Neuzil, C Zhang, J Pipper, S Oh, L Zhuo - Nucleic acids research, 2006