作者
Flavia Rodrigues da Silva, Mayara Rodrigues Brandão de Paiva, Lays Fernanda Nunes Dourado, Rummenigge Oliveira Silva, Carolina Nunes da Silva, Bruna Lopes da Costa, Cibele Rodrigues Toledo, Maria Elena de Lima, Armando da Silva-Cunha
发表日期
2018
期刊
The Journal of Venomous Animals and Toxins Including Tropical Diseases
卷号
24
出版商
Centro de Estudos de Venenos e Animais Peçonhentos-CEVAP
简介
Background
The great diversity of molecules found in spider venoms include amino acids, polyamines, proteins and peptides, among others. Some of these compounds can interact with different neuronal receptors and ion channels including those present in the ocular system. To study potential toxicity and safety of intravitreal injection in rabbits of LyeTx I b, a synthetic peptide derived from the toxin LyeTx I found in venom from the spider Lycosa eritrognatha and to evaluate the angiogenic activity on a CAM model.
Methods
ARPE-19 cells were treated with LyeTx I b (0.36; 0.54; 0.72; 2.89; 4.34 or 9.06 μM). In this study, New Zealand rabbits were used. LyeTx I b (2.89 μM) labeled with FITC dissolved in PBS, or only PBS, were injected into vitreous humor. Electroretinogram (ERG) was recorded 1 day before injection and at 7, 14 and 28 days post-injection. Clinical examination of the retina was conducted through …
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FR da Silva, MRB de Paiva, LFN Dourado, RO Silva… - The Journal of Venomous Animals and Toxins …, 2018