作者
Nenad Macesic, Jane Hawkey, Ben Vezina, Jessica A Wisniewski, Hugh Cottingham, Luke V Blakeway, Taylor Harshegyi, Katherine Pragastis, Gnei Zweena Badoordeen, Amanda Dennison, Denis W Spelman, Adam WJ Jenney, Anton Y Peleg
发表日期
2023
期刊
bioRxiv
页码范围
2023.03. 25.534241
出版商
Cold Spring Harbor Laboratory
简介
Infections caused by metallo-beta-lactamase-producing organisms (MBLs) are a global health threat. Our understanding of transmission dynamics and how MBLs establish endemicity remains limited. We analysed two decades of blaIMP-4 evolution in a hospital using sequence data from 270 clinical and environmental isolates (including 169 completed genomes) and identified extreme gene promiscuity across 7 Gram-negative genera, 68 bacterial strains and 7 distinct plasmid types. An initial multi-species outbreak of conserved IncC plasmids (95 genomes across 37 strains) allowed endemicity to be established through the ability of blaIMP-4 to disseminate in successful strain-genetic setting pairs we termed ‘propagators’, in particular Serratia marcescens and Enterobacter hormaechei. From this reservoir, blaIMP-4 persisted through diversification of genetic settings that resulted from transfer of blaIMP-4 plasmids between bacterial hosts and of the integron carrying blaIMP-4 between plasmids. Our findings provide a framework for understanding endemicity and spread of MBLs and may have broader applicability to other carbapenemase-producing organisms.
学术搜索中的文章
N Macesic, J Hawkey, B Vezina, JA Wisniewski… - bioRxiv, 2023