作者
Romney M Humphries, Daniel A Green, Audrey N Schuetz, Yehudit Bergman, Shawna Lewis, Rebecca Yee, Stephania Stump, Mabel Lopez, Nenad Macesic, Anne-Catrin Uhlemann, Peggy Kohner, Nicolynn Cole, Patricia J Simner
发表日期
2019/11
期刊
Journal of clinical microbiology
卷号
57
期号
11
页码范围
10.1128/jcm. 01269-19
出版商
American Society for Microbiology
简介
Susceptibility testing of the polymyxins (colistin and polymyxin B) is challenging for clinical laboratories. The Clinical and Laboratory Standards Institute (CLSI) Antimicrobial Susceptibility Testing Subcommittee evaluated two methods to enable accurate testing of these agents. These methods were a colistin broth disk elution (CBDE) and a colistin agar test (CAT), the latter of which was evaluated using two inoculum volumes, 1 μl (CAT-1) and 10 μl (CAT-10). The methods were evaluated using a collection of 270 isolates of Enterobacterales, 122 Pseudomonas aeruginosa isolates, and 106 Acinetobacter spp. isolates. Overall, 94.4% of CBDE results were in essential agreement and 97.9% in categorical agreement (CA) with reference broth microdilution MICs. Nine very major errors (VME; 3.2%) and 3 major errors (ME; 0.9%) were observed. With the CBDE, 98.6% CA was observed for Enterobacterales (2.5% VME …
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RM Humphries, DA Green, AN Schuetz, Y Bergman… - Journal of clinical microbiology, 2019