作者
Frances Weidert, Christina Von Roemeling, James McGuiness, Jonathan Chardon-Robles, Nagheme Thomas, Anna Devries, Sadeem Qdaisat, Dingpeng Zhang, Adam Grippin, Aida Karachi, Jianping Huang, Maryam Rahman, Elizabeth Ogando-Rivas, Paul Castillo, Eugene Hwang, Hector Mendez-Gomez, Natalie Silver, John A Ligon, Elias Sayour
发表日期
2023/11/1
来源
Journal for ImmunoTherapy of Cancer
卷号
11
期号
Suppl 1
出版商
BMJ Specialist Journals
简介
Background
Diffuse midline glioma (DMG) is a universal fatal glial brain cancer in children. We tested our novel multilamellar mRNA lipid particle aggregate vaccine (RNA-LPA, IND19304—Sayour),1 a tumor-agnostic treatment platform that encapsulates tumor specific RNA and delivers the payload in a highly immunogenic fashion, as an approach to treating this currently incurable cancer.
Methods
Using the K2 DMG model,2 we implant H3K27M-expressing DMG cells into the 4th ventricle of P1-P3 neonatal C57BL/6 mice. RNA-LPA generated from predicated human H3K27M epitopes or total-tumor mRNA are administered intravenously beginning at day 35. We performed multiparameter 3D geospatial fluorescent microscopy to characterize mRNA transduction. Immunologic responses to treatment were evaluated by multiparameter flow cytometry, microscopy, and cytokine profiling.
Results
Mice developed clinical …
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F Weidert, C Von Roemeling, J McGuiness… - 2023