作者
Alexander Assmann, Christofer Delfs, Hiroshi Munakata, Franziska Schiffer, Kim Horstkötter, Khon Huynh, Mareike Barth, Volker R Stoldt, Hiroyuki Kamiya, Udo Boeken, Artur Lichtenberg, Payam Akhyari
发表日期
2013/8/1
期刊
Biomaterials
卷号
34
期号
25
页码范围
6015-6026
出版商
Elsevier
简介
Decellularization is a promising option to diminish immune and inflammatory response against donor grafts. In order to accelerate the autologous in vivo recellularization of aortic conduits for an enhanced biocompatibility, we tested fibronectin surface coating in a standardized rat implantation model. Detergent-decellularized rat aortic conduits (n = 36) were surface-coated with covalently Alexa488-labeled fibronectin (50 μg/ml, 24 h) and implanted into the systemic circulation of Wistar rats for up to 8 weeks (group FN; n = 18). Uncoated implants served as controls (group C; n = 18). Fibronectin-bound fluorescence on both surfaces of the aortic conduits was persistent for at least 8 weeks. Cellular repopulation was examined by histology and immunofluorescence (n = 24). Luminal endothelialization was significantly accelerated in group FN (p = 0.006 after 8 weeks), however, local myofibroblast hyperplasia with …
学术搜索中的文章
A Assmann, C Delfs, H Munakata, F Schiffer… - Biomaterials, 2013