作者
Erica Dander, Adriana Balduzzi, Greta Zappa, Giovanna Lucchini, Paolo Perseghin, Valentina Andre, Elisabetta Todisco, Daoud Rahal, Maddalena Migliavacca, Daniela Longoni, Graziella Solinas, Antonello Villa, Emilio Berti, Pamela Della Mina, Matteo Parma, Paola Allavena, Ettore Biagi, Attilio Rovelli, Andrea Biondi, Giovanna D’Amico
发表日期
2009/12/15
期刊
Transplantation
卷号
88
期号
11
页码范围
1261-1272
出版商
LWW
简介
Objectives.
Graft-versus-host disease (GVHD) is a major obstacle to safe allogeneic hematopoietic stem-cell transplantation, leading to significant mortality. Recently, T-helper (TH)-17 cells have been shown to play a central role in mediating several autoimmune diseases. The aim of our study was to investigate the relationship between TH-17 cells and GVHD occurring in transplanted patients.
Methods.
Blood samples were collected from 51 hematopoietic stem-cell transplantation patients and 15 healthy donors. Patients with GVHD were monitored for the presence of TH-17 cells by ELISPOT or flow cytometry in the peripheral blood and by confocal microscopy in GVHD lesions. Cytokine plasma levels were detected by ELISA.
Results.
An increased TH-17 population (up to 4.8% of peripheral blood CD4+ T lymphocytes) was observed in patients with acute GVHD and (up to 2.4%) in patients with active chronic GVHD …
学术搜索中的文章
E Dander, A Balduzzi, G Zappa, G Lucchini… - Transplantation, 2009