作者
Birgit Huber, Kirsten Borchers, Günter EM Tovar, Petra J Kluger
发表日期
2016/1
期刊
Journal of biomaterials applications
卷号
30
期号
6
页码范围
699-710
出版商
SAGE Publications
简介
In vitro engineering of autologous fatty tissue constructs is still a major challenge for the treatment of congenital deformities, tumor resections or high-graded burns. In this study, we evaluated the suitability of photo-crosslinkable methacrylated gelatin (GM) and mature adipocytes as components for the composition of three-dimensional fatty tissue constructs. Cytocompatibility evaluations of the GM and the photoinitiator Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) showed no cytotoxicity in the relevant range of concentrations. Matrix stiffness of cell-laden hydrogels was adjusted to native fatty tissue by tuning the degree of crosslinking and was shown to be comparable to that of native fatty tissue. Mature adipocytes were then cultured for 14 days within the GM resulting in a fatty tissue construct loaded with viable cells expressing cell markers perilipin A and laminin. This work demonstrates that mature …
学术搜索中的文章
B Huber, K Borchers, GEM Tovar, PJ Kluger - Journal of biomaterials applications, 2016