作者
William Nelson, Meizhong Luo, Jianxin Ma, Matt Estep, James Estill, Ruifeng He, Jayson Talag, Nicholas Sisneros, David Kudrna, HyeRan Kim, Jetty SS Ammiraju, Kristi Collura, Arvind K Bharti, Joachim Messing, Rod A Wing, Phillip SanMiguel, Jeffrey L Bennetzen, Carol Soderlund
发表日期
2008/12
期刊
BMC genomics
卷号
9
页码范围
1-16
出版商
BioMed Central
简介
Background
Many plant genomes are resistant to whole-genome assembly due to an abundance of repetitive sequence, leading to the development of gene-rich sequencing techniques. Two such techniques are hypomethylated partial restriction (HMPR) and methylation spanning linker libraries (MSLL). These libraries differ from other gene-rich datasets in having larger insert sizes, and the MSLL clones are designed to provide reads localized to "epigenetic boundaries" where methylation begins or ends.
Results
A large-scale study in maize generated 40,299 HMPR sequences and 80,723 MSLL sequences, including MSLL clones exceeding 100 kb. The paired end reads of MSLL and HMPR clones were shown to be effective in linking existing gene-rich sequences into scaffolds. In addition, it was shown that the MSLL clones can be used for anchoring …
学术搜索中的文章
W Nelson, M Luo, J Ma, M Estep, J Estill, R He, J Talag… - BMC genomics, 2008