作者
Ji-Li Chen, Anthony J Morgan, Guillaume Stewart-Jones, Dawn Shepherd, Giovanna Bossi, Linda Wooldridge, Sarah L Hutchinson, Andrew K Sewell, Gillian M Griffiths, P Anton van der Merwe, E Yvonne Jones, Antony Galione, Vincenzo Cerundolo
发表日期
2010/2/15
期刊
The journal of immunology
卷号
184
期号
4
页码范围
1829-1839
出版商
American Association of Immunologists
简介
Although several cancer immunotherapy strategies are based on the use of analog peptides and on the modulation of the TCR affinity of adoptively transferred T cells, it remains unclear whether tumor-specific T cell activation by strong and weak TCR stimuli evoke different Ca 2+ signatures from the Ca 2+ intracellular stores and whether the amplitude of Ca 2+ release from the endoplasmic reticulum (ER) can be further modulated by coreceptor binding to peptide/MHC. In this study, we combined functional, structural, and kinetic measurements to correlate the intensity of Ca 2+ signals triggered by the stimulation of the 1G4 T cell clone specific to the tumor epitope NY-ESO-1 157–165. Two analogs of the NY-ESO-1 157–165 peptide, having similar affinity to HLA-A2 molecules, but a 6-fold difference in binding affinity for the 1G4 TCR, resulted in different Ca 2+ signals and T cell activation. 1G4 stimulation by the …
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JL Chen, AJ Morgan, G Stewart-Jones, D Shepherd… - The journal of immunology, 2010