作者
Michael J Palmowski, Ed Man-Lik Choi, Ian F Hermans, Sarah C Gilbert, Ji-Li Chen, Uzi Gileadi, Mariolina Salio, Aline Van Pel, Stephen Man, Eivor Bonin, Peter Liljestrom, P Dunbar, Vincenzo Cerundolo
发表日期
2002/5/1
期刊
The Journal of Immunology
卷号
168
期号
9
页码范围
4391-4398
出版商
American Association of Immunologists
简介
Recombinant vaccines encoding strings of virus-or tumor-derived peptides and/or proteins are currently being designed for use against both cancer and infectious diseases. These vaccines aim to induce cytotoxic immune responses against several Ags simultaneously. We developed a novel tetramer-based technique, based on chimeric HLA A2/H-2K b H chains, to directly monitor the CTL response to such vaccines in HLA-A2 transgenic mice. We found that priming and boosting with the same polyepitope construct induced immune responses that were dominated by CTL of a single specificity. When a mixture of viruses encoding single proteins was used to boost the polyepitope primed response, CTL of multiple specificities were simultaneously expanded to highly effective levels in vivo. In addition, we show that a preexisting response to one of the epitopes encoded within a polyepitope construct significantly …
引用总数
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MJ Palmowski, EML Choi, IF Hermans, SC Gilbert… - The Journal of Immunology, 2002