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High-resolution (200 × 200 × 1,500 μm³) imaging was performed to derive quantitative cerebral blood flow (CBF) and apparent diffusion coefficient (ADC) maps in stroke rats (permanent occlusion) every 30 minutes up to 3 hours after occlusion onset, followed by histology at 24 hours. An improved automated iterative-self-organizing-data-analysis-algorithm (ISODATA) was developed to dynamically track ischemic tissue fate on a pixel-by-pixel basis during the acute phase. ISODATA-resolved clusters were overlaid on the CBF-ADC scatterplots and image spaces. Tissue volume ADC, and CBF of each ISODATA cluster were derived. In contrast to the single-cluster normal left hemisphere (ADC = 0.74±0.02 × 10⁻³ mm²/s, CBF = 1.36±0.22 mL g⁻¹ min⁻¹, mean±SD, n = 8), the right ischemic hemisphere exhibited three ISODATA clusters, namely: “normal” (normal ADC and CBF), “ischemic core” (low CBF and ADC …