作者
Pieter BAA Van Driel, Mark C Boonstra, Henriette AJM Prevoo, Martijn van de Giessen, TJA Snoeks, QRJG Tummers, S Keereweer, RA Cordfunke, A Fish, JDH Van Eendenburg, BPF Lelieveldt, J Dijkstra, CJH van de Velde, PJK Kuppen, AL Vahrmeijer, CWGM Löwik, CFM Sier
发表日期
2016/12
期刊
BMC cancer
卷号
16
页码范围
1-13
出版商
BioMed Central
简介
Background
Evaluation of resection margins during cancer surgery can be challenging, often resulting in incomplete tumour removal. Fluorescence-guided surgery (FGS) aims to aid the surgeon to visualize tumours and resection margins during surgery. FGS relies on a clinically applicable imaging system in combination with a specific tumour-targeting contrast agent. In this study EpCAM (epithelial cell adhesion molecule) is evaluated as target for FGS in combination with the novel Artemis imaging system.
Methods
The NIR fluorophore IRDye800CW was conjugated to the well-established EpCAM specific monoclonal antibody 323/A3 and an isotype IgG1 as control. The anti-EpCAM/800CW conjugate was stable in serum and showed preserved binding capacity as evaluated on EpCAM positive and negative cell lines, using flow cytometry and cell …
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PBAA Van Driel, MC Boonstra, HAJM Prevoo… - BMC cancer, 2016