查看文章

Through genetic recombination, the adaptive immune system generates a diverse T cell repertoire allowing recognition of a vast spectrum of foreign antigens. Any given CD8⁺ T cell specificity is thought to be rare, but none have been directly quantified. Here, major histocompatibility complex tetramer and magnetic-bead technology were coupled to quantitate naive antigen-specific CD8⁺ T cells and the early response to infection. Among six specificities measured, the number of naive antigen-specific precursors ranged from ∼80 to 1200 cells/mouse. After vesicular stomatitis virus infection, the antigen-specific CD8⁺ T cell response occurred in discrete phases: prolonged activation of a subset of cells over the first 72 hr followed by a rapid proliferative burst. Naive precursor frequency altered response kinetics and regulated immunodominance, as well as the time required for the responding population to shift toward …