作者
George X Huang, Nils R Hallen, Minkyu Lee, Kelly Zheng, Xin Wang, Michael V Mandanas, Sarah Djeddi, Daniela Fernandez, Jonathan Hacker, Tessa Ryan, Regan W Bergmark, Neil Bhattacharyya, Stella Lee, Alice Z Maxfield, Rachel E Roditi, Kathleen M Buchheit, Tanya M Laidlaw, James E Gern, Teal S Hallstrand, Anuradha Ray, Sally E Wenzel, Joshua A Boyce, Maria Gutierrez-Arcelus, Nora A Barrett
发表日期
2023/10/17
期刊
bioRxiv
出版商
Cold Spring Harbor Laboratory Preprints
简介
Background
The airway epithelium plays a central role in the pathogenesis of chronic respiratory diseases such as asthma and chronic rhinosinusitis with nasal polyps (CRSwNP), but the mechanisms by which airway epithelial cells (EpCs) maintain inflammation are poorly understood.
Objective
We hypothesized that transcriptomic assessment of sorted airway EpCs across the spectrum of differentiation would allow us to define mechanisms by which EpCs perpetuate airway inflammation.
Methods
Ethmoid sinus EpCs from adult patients with CRS were sorted into 3 subsets, bulk RNA sequenced, and analyzed for differentially expressed genes and pathways. Single cell RNA-seq (scRNA-seq) datasets from eosinophilic and non-eosinophilic CRSwNP and bulk RNA-seq of EpCs from mild/moderate and severe asthma were assessed. Immunofluorescent staining and ex vivo functional analysis of sinus EpCs were used …
学术搜索中的文章
GX Huang, NR Hallen, M Lee, K Zheng, X Wang… - bioRxiv, 2023