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Background:

Colorectal cancer (CRC) is one of the leading causes of mortality and morbidity. The Kashmir valley, in Northern India, has been described as a high-risk area for colorectal cancer.

Aim:

The aim was to make a preliminary attempt to study mutations in exons 5–8 (the DNA binding domain) of the tumour suppressor gene TP53 in 42 CRC patients from Kashmir.

Materials and methods:

The study population consisted of 42 patients diagnosed with colorectal cancer. Mutations in exons 5–8 of the TP53 gene were detected by means of single-strand conformation polymorphism (SSCP). All samples that showed different band migration patterns in the SSCP were confirmed by sequencing.

Results:

The 28 mutations were found in the TP53 gene in 19 patients, comprised 23 substitutions (17 transitions+ six transversions), and five insertions. The 23 substitutions represent 18 missense mutations, leading to amino …