查看文章

A computerised approach to the structural analysis of unbranched regular polysaccharides is described, which is based on an evaluation of the ¹³C-n.m.r. spectra for all possible primary structures within the additive scheme starting from the chemical shifts of the ¹³C resonances of the constituent monosaccharides and the average values of the glycosylation effects. The analysis reveals a structure (or structures), the evaluated spectrum of which resembles most closely that observed. The approach has been verified by using a series of bacterial polysaccharides of known structure and, in combination with methylation analysis data, for the determination of the presently unknown structures of the O-specific polysaccharides from Salmonella arizonae O59 and O63, and Proteus hauseri O19.