作者
Daniel Y Li, Albert Busch, Hong Jin, Ekaterina Chernogubova, Jaroslav Pelisek, Joakim Karlsson, Bengt Sennblad, Shengliang Liu, Shen Lao, Patrick Hofmann, Alexandra Bäcklund, Suzanne M Eken, Joy Roy, Per Eriksson, Brian Dacken, Deepak Ramanujam, Anne Dueck, Stefan Engelhardt, Reinier A Boon, Hans-Henning Eckstein, Joshua M Spin, Philip S Tsao, Lars Maegdefessel
发表日期
2018/10/9
期刊
Circulation
卷号
138
期号
15
页码范围
1551-1568
出版商
Lippincott Williams & Wilkins
简介
Background
Long noncoding RNAs have emerged as critical molecular regulators in various biological processes and diseases. Here we sought to identify and functionally characterize long noncoding RNAs as potential mediators in abdominal aortic aneurysm development.
Methods
We profiled RNA transcript expression in 2 murine abdominal aortic aneurysm models, Angiotensin II (ANGII) infusion in apolipoprotein E–deficient (ApoE−/−) mice (n=8) and porcine pancreatic elastase instillation in C57BL/6 wild-type mice (n=12). The long noncoding RNA H19 was identified as 1 of the most highly upregulated transcripts in both mouse aneurysm models compared with sham-operated controls. This was confirmed by quantitative reverse transcription–polymerase chain reaction and in situ hybridization.
Results
Experimental knock-down of H19, utilizing site-specific antisense oligonucleotides (LNA-GapmeRs) in …
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DY Li, A Busch, H Jin, E Chernogubova, J Pelisek… - Circulation, 2018